The division of biotechnological drug engineering is constituted of seven research groups, which have more than thirty staff members including nine core members. the aim of the division is to accelerate research and development of original biotechnological drugs and biosimilar medicines for the treatment or prevention of major diseases and infectious diseases. Through the advantage of integration of multiple areas of biotechnological drug research, the division is able to conduct preclinical research with high efficiency.
Prof. Rui-tian Liu’s group developed a novel nanoparticle, which is conjugated with CD47 extracellular domain via reactive oxygen species (ROS)-responsive phenylborate
ester bond and BBB penetrating peptide (CRT), with microglia modulation agent Nec-1s encapsulated in it. The results of this study prove for the first time that the conditionally released “don’t eat me” CD47 signal significantly facilitates the drug delivery to microglia and has a good therapeutic effect on Alzheimer’s disease (AD) transgenic mice. This microglia-targeted drug delivery nanoparticle warrants further study for AD treatment (Advanced Functional Materials, DOI: 10.1002/adfm.201910691).
Aiming at the emergency need of COVID-19, professor Lei Zhou’s team developed antibody and nucleic acid detection kits by relying on the nano material, reagent system and drying process on the premise of capacity to response to public health emergency, and they got CE certification. About the COVID-19 nucleic acid detection kit, they achieved that all-reagent prestaging, could be stored and transferred under room temperature. About the antibody detection kit, fingertip blood could be directly used as sample and the result is no more than 15 minutes.
Prof. Gui-feng Zhang’s group prepares novel scaffolds for the culture liver cell line, by modifying polyvinyl alcohol (PVA) scaffold with collagen. Glutaraldehyde, KH-550 and native type I collagen were used to modify PVA 3D scaffold to establish a suitable composite scaffold for hepatocyte culture. The coating of collagen on PVA scaffolds promoted hydrophilicity and HepG2 cell adherence. Porous three-dimensional scaffolds formed from natural biopolymers collagen and synthetic polymer PVA that are chemically cross-linked by KH-550 and glutaraldehyde have shown improved hepatocyte adhesion and functions of the hepatocytes （Journal of Biomaterials Applications，DOI：10.1177/0885328220933505）.
Prof. Songping Zhang’s team established a new technology based on capillary zone electrophoresis (CZE) for the separation and quantitative detection of 146S in monovalent and bivalent FMDV. They analyzed the difference in the ratio of charge to mass of 146S in different polyvalent serotypes. This technique has been successfully applied to the antigenic quantification of a commercial multivalent FMDV vaccine without interference from nucleic acid impurities.
Fig.1 A conditionally releasable “Do not Eat Me” CD47 signal facilitates microglia-targeted drug delivery for the treatment of Alzheimer’s Disease.
Fig.2 COVID-19 Nucleic acid Detection Reagent (left), COVID-19 Antibody Detection Reagent (middle), CE Certification (right)
Fig.3 Three dimensional polyvinyl alcohol scaffolds modified with collagen for HepG2 cell culture.
Fig.4 On-line separation and quantification of virus antigens of different serotypes in multivalent foot-and-mouth disease virus vaccines by capillary zone electrophoresis.